Table 2. Gas production and parameters after in vitro incubation of treatments using mixed rumen fluid

Item	CNSL (%)					SEM	p-value
	0	0.02	0.2	1	2		Linear	Quadratic
Gas production (mL/g DM)
3 h	62.7ab	59.8b	62.2ab	63.4ab	65.1a	0.82	< 0.05	0.6132
6 h	102	96.9	101	104	104	2.10	0.0815	0.4180
9 h	143	136	143	146	145	3.39	0.1790	0.3559
12 h	176	169	176	180	177	3.96	0.2915	0.2644
24 h	263	256	261	260	245	4.16	0.0213	0.0980
36 h	312a	303a	306a	299a	277b	4.63	< 0.001	0.1095
48 h	333a	325a	326a	318a	294b	4.55	< 0.001	0.0945
Fitted parameters of gas
Vmax1)	356a	350ab	347ab	333b	300c	5.08	< 0.001	0.1230
Kg2)	0.06c	0.06c	0.06bc	0.06b	0.07a	0.001	< 0.001	0.5022
Methane production
%	11.6ab	11.8a	11.8a	11.4b	10.6c	0.06	< 0.001	0.0677
mL/g DM	38.8a	38.2ab	38.4ab	36.2b	31.2c	0.55	< 0.001	< 0.05
mL/g DDM	66.7a	65.8ab	60.3ab	62.4ab	51.0b	3.30	< 0.05	0.1559

Theoretical maximum gas production (mL/g DM).
Fractional rate of gas production (h⁻¹).
Means within a row with different superscript letters indicate significant differences among the treatments (p < 0.05).