Table 3. Summary of the studies evaluating effects of 3-NOP on ruminal methane reduction methane

Study no.	Authors	Year	Animal	3-NOP level (mg/kg DMI)	Methane reduction (% of control)	References
1	Martínez-Fernández et al.	2014	Sheep	0, 111.2	16–19	[55]
2	Romero-Perez et al.	2014	Beef cattle	0, 47.4, 143.6, 304.9	4–38	[56]
3	Romero-Perez et al.	2015	Beef cattle	0, 280.1	59	[53]
4	Vyas et al.	2016	Beef cattle	0, 100, 200	17–38	[57]
5	Vyas et al.	2018	Beef cattle	0, 50, 75, 100, 150, 200	18-10	[58]
6	Vyas et al.	2018	Beef cattle	0, 125, 200	41–42	[59]
7	Martinez-Fernandez et al.	2018	Beef cattle	0, 337.8	30	[51]
8	Kim et al.	2019	Beef cattle	0, 100	17	[60]
9	Haisan et al.	2014	Dairy cow	0, 129.5	60	[61]
10	Reynolds et al.	2014	Dairy cow	0, 26.6, 135.1	10-7	[62]
11	Hristov et al.	2015	Dairy cow	0, 40.0, 60.0, 80.0	24–33	[63]
12	Lopes et al.	2016	Dairy cow	0, 60.0	31	[52]
13	Haisan et al.	2017	Dairy cow	0, 68.3, 132.3	23–37	[64]
14	Van Wesemael et al.	2019	Dairy cow	0, 71.7, 75.1	23–28	[65]

3-NOP, 3-nitrooxypropanol; DMI, dry matter intake.