Table 1. Formula and chemical compositions of basal diet

Items	Basal diet (control)
Ingredient (%)
Corn	60.48
Soybean meal (45%)	11.00
Rapeseed meal	5.50
Corn gluten meal	2.16
DDGS	6.65
Animal fat	1.50
Lysine-sulfate (55%)	0.33
DL-methionine (99%)	0.15
MCP	0.62
Limestone	10.50
Salt	0.25
Vitamin premix1)	0.10
Mineral premix2)	0.20
Choline-Cl (50%)	0.03
Phytase	0.03
NaHCO3	0.50
Total	100.0
Calculated values (%)
CP	15.50
Ca	4.10
Avail. P	0.30
Total Lys	0.84
Total TSAA	0.72
AMEn (kcal/kg)	2,740

Vitamin mixture provided the following nutrients per kg of basal diet: vitamin A, 10,000 IU; vitamin D₃, 3,500 IU; vitamin E, 10 mg; vitamin K₃, 3 mg; thiamine, 2 mg; riboflavin, 6 mg; vitamin B₆, 4 mg; pantothenic acid, 10 mg; folic acid, 0.82 mg; niacin, 30 mg; vitamin B₁₂, 0.0235 mg.
Mineral mixtures provided the following nutrients per kg of diets: Mn, 20 mg; Zn, 45 mg; Fe, 50 mg; Cu, 10 mg; Co, 0.15 mg; Se, 0.10 mg.
DDGS, dried distillers grains with solubles; MCP, monocalcium phosphate; TSAA, total sulfur amino acid.